Fertility & oxidative stress:Pre-clinical evidences that antioxidant supplementation corrects sperm DNAoxidative damage and improves reproductive success

drevet

Prof. Joël Drevet from INSERM U1103-Clermont University, France asked the question "Does a novel antioxidant formulation designed to treat male infertility associated with oxidative stress: promising preclinical evidence from animal models?".


By using immunocytochemical detection of8OHdGas a biomarker ofDNAoxidation, analysis of control mice revealed that around 30% of the sperm population was positively stained. This level increased to about 60% in transgenic mice deficient in the antioxidant enzyme, GPX5. Our results indicate that an 8 week pretreatment of Gpx5 KO mice with the antioxidant formulation provided complete protection of sperm DNA against oxidative damage. In mouse models of scrotal heat stress, only 35% (19/54) of female mice became pregnant resulting in 169 fetuses with 18% fetal resorption (30/169). This is in contrast to the antioxidant pretreated group where 74% (42/57) of female mice became pregnant, resulting in 427 fetuses with 9% fetal resorption (38/427). In both animal models the protection provided by the novel antioxidant was statistically significant (P , 0.01 for the reduction of 8OHdG in the spermatozoa of Gpx5 KOmice and P , 0.05 for increase in fertility in the scrotal heat stress model).